A good incubator is key in
the successful artificial incubation of tree python eggs. Over the last
few years I've engineered, designed, and tested a number of incubator
designs... some failed miserably, others produced marginal results, and
a few did very well. Tree python eggs are pretty unique, and provide
a particularly interesting challenge to the keeper. Although I've
traditionally used vertically oriented incubators constructed of
melamine or some similar material, recent market introductions of
cooler-based units caught my attention and I thought I'd try my hand at
building a unit that would satisfy my needs.
|
|
|
|
|
| Since
the egg boxes are, in my opinion, one of the most important
components of a good incubator, I'm always been on the lookout
for boxes I think will work well. I recently found some solidly
constructed clear polycarbonate boxes. They're made by
Cambro for the restaurant industry and are designed for use in food
storage. They're available with drain shelves that work
excellently for no-substrate egg incubation, and can be
purchased with domed
lids. The domed lids all but eliminate the potential for egg
loss due to dripping condensation. Unlike flat lids where
condensation can drip into the deli-cups and onto the eggs, the
condensation that forms on the domed lids runs down the sides of
the lid and back into the tray. The domed lids are manufactured by
Carlisle Foodservice Products. The lids and boxes come in a
myriad of sizes, but I use ½ size boxes that are approximately
12" X 10" X 4". The ½ size box easily
accommodates 4 deli cups that hold 6 or 7 eggs each. With the
addition of vinyl
bumpers, a 1/8" gap can be created between lid and base
allowing for adequate gas exchange and thermocouple access
during incubation.
I like to put my
eggs in 12 oz. deli-cups. They prevent the eggs from rolling
should the incubator be jarred during incubation but still allow
for adequate humidity and air flow. I cut clear rubber mesh to
fit in the bottom of the deli-cups. It serves to lift the eggs
slightly off the bottom of the cup in order to ward off
potential condensation problems and, to a lesser extent, helps
promote healthy air flow.
|
|
 |
 |
| Box
and lid |
Drain
shelf |
|
 |
| Box,
drain shelf, and domed lid |
Vinyl
bumpers |
 |
 |
| Deli
cup with rubber mesh liner |
Four
cups in egg box |
| After selecting my
egg boxes, I looked for a cooler that would accommodate them. I
settled on a 120 quart Igloo marine cooler. They can be had for
relatively little money and provide an intuitive platform for a
solid incubator. The 120 quart model will hold three egg boxes
and potentially as many as 84 eggs at one time. The drain port
provides ample access for the introduction of thermocouple
probes, proportional thermostat probes, heating element cords,
and air lines. |

|
|
Igloo
120 quart marine cooler |
| I use a
Cooper SH66A Industrial Thermistor Thermocouple Thermometer to
measure temperatures. The unit is calibrated to standards
traceable to the National Institute of Standards and Technology.
I had the unit calibrated at 87°F and use three specially built
probes that were calibrated with the unit to provide exceptional
accuracy. The unit is marketed as having an accuracy of +/-
0.3°F but my unit lab tested to a 0.1°F error rate. The unit
can run three probes simultaneously which helped immensely
during my incubator testing simply because I'm running three egg
boxes per cooler and this allowed me to run testing with a probe
in each box. I purchased the unit from Tech
Instrumentation. |
 |
| Cooper
SH66A Electro-therm |
| I choose to heat my
incubators with EasyHeat
HB freeze protection cable. It's sealed, and works great in
a high humidity environment. For the 120 quart cooler, I use the
HB05 115 watt, 16.41' cable. The cable is situated in the bottom
of the cooler, wrapped in some fashion around a PVC
frame or metal shelf so as to keep the cable evenly distributed
and safely arranged (coils of a heat cable should not be allowed
to contact one another in order to avoid high heat build-up, and
the failure resulting from the consequential melting of the
protective insulation). I wrap mine through a section of coated
wire shelving that was cut from its original 36" length
down to 32" in order to fit in the cooler. |
 |
| I use a Helix Basic
System Proportional Thermostat for my incubators. In my
experience, they're accurate, reliable, effective, and have an
adjustability that suits my needs. Recently, Spyder robotics has
introduced a new proportional thermostat into the market and
although it looks to be very nice and will most likely work very
well for the purpose of incubating eggs, I have not had the
opportunity to test one personally. |
| Preliminary
Designs |
| |
|
My first designs were very basic. There was a heating element,
several water trays, and a shelf that held the egg boxes. It
seemed straight-forward enough, but tests revealed a number of
hot and cold spots throughout the cooler. No matter how I
rearranged the contents I could not prevent variances in egg box
temperatures. Heat plumes formed around the periphery of the
water trays (where rising heat was trying to move toward the
upper levels of the incubators' interior) and were heating the
egg boxes differentially. The effect lessened, but did not
resolve itself even when the incubator stabilized at its target
temperature. It became clear that I needed to incorporate some
sort of fan in order to deal with the issue.
In my later designs, I built an air exchange manifold with an inline
fan in order to move air from the bottom of the incubator to the
top (or vise versa depending on what turned out to be most successful).
I chose 40mm 12V brushless CPU fans for the job. The fans run
quietly and with very little vibration. The fans are wired
directly to a 12V transformer. I built the fan
housing from a 1½" PVC coupling and two
¾"-1½" flush reducing bushings. I milled 40mm X 20mm
holes in the 1½" coupling for motor placement with a
spiral cutting bit and a Dremel. The
idea and design were partially effective. The manifold was successful at
moving air, but it became clear that moving air along the
vertical gradient was doing little to solve my temperature
variance problem. I tried a multitude of configurations but none
resulted in exceptional results. Some configurations were
somewhat stable and probably could have worked successfully, but
I didn't want to take the chance. |
|
 |
|
Drain
port access showing thermocouple probes (red),
proportional thermostat probe (white), air hose (blue), and heat
cable (orange and black) |
|
 |
 |
|
Fan
housing, manifold, and egg crate shelf |
Close-up
of fan housing and manifold |
|
 |
|
|
Manifold
and fan housing |
12V
7cfm fan |
|
After nearly 45 days of testing, I changed directions a little
and stumbled across a design that has turned out to be quite
remarkable. Initial testing revealed several qualities that made
the configuration ideal. First, it maintained identical and
stable temperatures in each egg box. Second, it allowed for the
system to achieve temperature gradually (and not exceed it). Third, it incorporated
the introduction of fresh air into the system and lastly, it
maintained saturation-level humidity.
I started with the
32" wire coated rack as a base. I built a frame out of
¾" PVC that fit beneath the wire rack. The heating cable
was wrapped around the PVC frame so that it was distributed
evenly along the length of the frame. The wire rack prevented
the heat cable from being damaged. On the wire rack
sit (3) 6 quart 12" x 10" x 4" stainless steel
pans filled with water. The stainless steel serves to conduct
heat more effectively for even heating and the water served two
purposes... to help humidify the incubator, and provide thermal
mass to aid in temperature stability.
One of the things
about traditional artificial incubation that's always bothered
me is gas exchange. Incubating eggs are metabolizing stored
energy and releasing waste gases. Most traditional egg
incubation strategies focus on maintaining ideal temperatures and
humidity and little attention seems to concentrate on fresh air exchange.
On an experimental basis I opted to include some form of fresh
air introduction into my incubator designs. For this purpose I
opted to use an aquarium air pump (Rena Air model 100). In my early designs the air
line ran into the intake manifold where the fresh air mixed with
warm internal air before being blown back into the incubator
through the exhaust portion of the manifold. Since the manifold
proved to be only marginally effective in the testing phase, I
reevaluated and bettered my design. I ultimately ran the air
line into the center stainless steel water tray and attached an air stone to it.
In addition to providing fresh air, the design helped boost the
humidity the last several often difficult to achieve percentage
points to total saturation.
The egg boxes sit on styrene egg crate positioned atop a PVC frame that separates it from the stainless steel water pans.
It's important that there's a separation between the boxes and
the water in order to aid in air flow beneath the egg boxes and
to eliminate the presence of hot spots in the incubator. On the
underside of the egg crate are fastened two 12V 40mm X 40mm X
20mm CPU fans. I zip tie them to the egg crate and position them
to create air flow parallel to the egg crate's surface. They
each move approximately 7 cubic feet of air per minute and
between the two of them, manage to completely homogenize the air
in the incubator... in regard to both temperature and humidity.
It's important to
note that I built this incubator with the intent of using it
with a consistent temperature regimen. Because I was
anticipating a number of clutches within a narrow timeframe, it wasn't particularly
feasible for me to use the 1-5-1, 30.5°C- 31.5°C- 30.0°C
regimen. The difference in incubator configuration between the
two techniques can be dramatic. A uniform temperature regimen
requires consistent ambient incubator air temperatures and the
1-5-1 regimen requires consistent egg surface
temperatures. Therefore, the placement of the probe is
CRITICAL to achieving an effective incubator. In my final cooler
incubator design, the proportional thermostat probe is placed in
the airspace underneath the egg boxes where it can sample the
fan-mixed air. If the probe is placed inside the egg box
(insulated from the ambient air in the incubator), disaster can
result. A probe interior to an egg box will send a message to
the thermostat to continue providing power to the heat source
even after the target temperature is reached inside the
incubator. When that happens, the inside of the incubator
continues to heat until the egg box reaches the ideal
temperature. At that time, the thermostat decreases power to the
heating element. Unfortunately, air in the incubator will have
already surpassed the target temperature and will continue to
heat the egg box until equilibrium is reached. A cyclical
increase and decrease in temperature will occur until final
equilibrium occurs. My tests indicated that this can mean
surpassing ideal temperatures by as much as 4°F and the
initiation of a fluctuation cycle that can last as long as two
hours after the incubator is closed. In the scenario where the
probe is exterior to the egg box, the thermostat decreases power
to the heater when the ambient air in the incubator reaches
target. The thermostat maintains that temperature and the egg
box gradually reaches target, and once equilibrium is reached,
the system maintains perfectly.
I am continuing to
test ideas that build upon the design that has thus far been my
most successful. Space limitations have kept me from being able
to utilize the domed covers in an effective design but work
continues... I'll post any effective developments as they occur.
|
|
 |
|
 |
| Wire
Shelf and PVC Frame |
Heat
tape wrapped around PVC frame |
|
|
| Heat
tape & PVC frame under plastic-coated wire shelf
|
Stainless
Steel Water Pans |
 |
 |
| PVC
Spacer Frame |
Fan
With Spliced Extensions |
 |
 |
|
Mounted
Fan
|
Mounted
Fan
|
 |
 |
| Fans
and Egg Crate Installed |
Air
Flow Diagram |
 |
 |
| Egg
Crate and Egg Boxes |
Incubator
With Installed Acrylic Interior Lids |
 |
 |
| Incubator
Testing |
Deli-cups
in boxes |
 |
 |
| nesting
container |
Sarlo
in her nest box |
 |
 |
| Sarlo
laying her eggs 3 March 2005 |
Sarlo
in her nest box 4 March 2005 |
 |
|
| Sarlo's
eggs 4 March 2005 |
|
|
|