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Incubator Design

 

   A good incubator is key in the successful artificial incubation of tree python eggs. Over the last few years I've engineered, designed, and tested a number of incubator designs... some failed miserably, others produced marginal results, and a few did very well. Tree python eggs are pretty unique, and provide a particularly interesting challenge to the keeper. Although I've traditionally used vertically oriented incubators constructed of melamine or some similar material, recent market introductions of cooler-based units caught my attention and I thought I'd try my hand at building a unit that would satisfy my needs.
 
   Since the egg boxes are, in my opinion, one of the most important components of a good incubator, I'm always been on the lookout for boxes I think will work well. I recently found some solidly constructed clear polycarbonate boxes. They're made by Cambro for the restaurant industry and are designed for use in food storage. They're available with drain shelves that work excellently for no-substrate egg incubation, and can be purchased with domed lids. The domed lids all but eliminate the potential for egg loss due to dripping condensation. Unlike flat lids where condensation can drip into the deli-cups and onto the eggs, the condensation that forms on the domed lids runs down the sides of the lid and back into the tray. The domed lids are manufactured by Carlisle Foodservice Products. The lids and boxes come in a myriad of sizes, but I use ½ size boxes that are approximately 12" X 10" X 4". The ½ size box easily accommodates 4 deli cups that hold 6 or 7 eggs each. With the addition of vinyl bumpers, a 1/8" gap can be created between lid and base allowing for adequate gas exchange and thermocouple access during incubation.

   I like to put my eggs in 12 oz. deli-cups. They prevent the eggs from rolling should the incubator be jarred during incubation but still allow for adequate humidity and air flow. I cut clear rubber mesh to fit in the bottom of the deli-cups. It serves to lift the eggs slightly off the bottom of the cup in order to ward off potential condensation problems and, to a lesser extent, helps promote healthy air flow.

 

    

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Box and lid Drain shelf
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Box, drain shelf, and domed lid Vinyl bumpers
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Deli cup with rubber mesh liner Four cups in egg box

  

   After selecting my egg boxes, I looked for a cooler that would accommodate them. I settled on a 120 quart Igloo marine cooler. They can be had for relatively little money and provide an intuitive platform for a solid incubator. The 120 quart model will hold three egg boxes and potentially as many as 84 eggs at one time. The drain port provides ample access for the introduction of thermocouple probes, proportional thermostat probes, heating element cords, and air lines.

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Igloo 120 quart marine cooler

 

   I use a Cooper SH66A Industrial Thermistor Thermocouple Thermometer to measure temperatures. The unit is calibrated to standards traceable to the National Institute of Standards and Technology. I had the unit calibrated at 87°F and use three specially built probes that were calibrated with the unit to provide exceptional accuracy. The unit is marketed as having an accuracy of +/- 0.3°F but my unit lab tested to a 0.1°F error rate. The unit can run three probes simultaneously which helped immensely during my incubator testing simply because I'm running three egg boxes per cooler and this allowed me to run testing with a probe in each box. I purchased the unit from Tech Instrumentation. SH66Awop.jpg (44778 bytes)
Cooper SH66A Electro-therm

 

   I choose to heat my incubators with EasyHeat HB freeze protection cable. It's sealed, and works great in a high humidity environment. For the 120 quart cooler, I use the HB05 115 watt, 16.41' cable. The cable is situated in the bottom of the cooler, wrapped in some fashion around a PVC frame or metal shelf so as to keep the cable evenly distributed and safely arranged (coils of a heat cable should not be allowed to contact one another in order to avoid high heat build-up, and the failure resulting from the consequential melting of the protective insulation). I wrap mine through a section of coated wire shelving that was cut from its original 36" length down to 32" in order to fit in the cooler. ezheat.jpg (53357 bytes)

 

   I use a Helix Basic System Proportional Thermostat for my incubators. In my experience, they're accurate, reliable, effective, and have an adjustability that suits my needs. Recently, Spyder robotics has introduced a new proportional thermostat into the market and although it looks to be very nice and will most likely work very well for the purpose of incubating eggs, I have not had the opportunity to test one personally.

Preliminary Designs
 
   My first designs were very basic. There was a heating element, several water trays, and a shelf that held the egg boxes. It seemed straight-forward enough, but tests revealed a number of hot and cold spots throughout the cooler. No matter how I rearranged the contents I could not prevent variances in egg box temperatures. Heat plumes formed around the periphery of the water trays (where rising heat was trying to move toward the upper levels of the incubators' interior) and were heating the egg boxes differentially. The effect lessened, but did not resolve itself even when the incubator stabilized at its target temperature. It became clear that I needed to incorporate some sort of fan in order to deal with the issue.

   In my later designs, I built an air exchange manifold with an inline fan in order to move air from the bottom of the incubator to the top (or vise versa depending on what turned out to be most successful). I chose 40mm 12V brushless CPU fans for the job. The fans run quietly and with very little vibration. The fans are wired directly to a 12V transformer. I built the fan housing from a 1½" PVC coupling and two ¾"-1½" flush reducing bushings. I milled 40mm X 20mm holes in the 1½" coupling for motor placement with a spiral cutting bit and a Dremel. The idea and design were partially effective. The manifold was successful at moving air, but it became clear that moving air along the vertical gradient was doing little to solve my temperature variance problem. I tried a multitude of configurations but none resulted in exceptional results. Some configurations were somewhat stable and probably could have worked successfully, but I didn't want to take the chance. 

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Drain port access showing thermocouple probes (red), proportional thermostat probe (white), air hose (blue), and heat cable (orange and black)
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Fan housing, manifold, and egg crate shelf Close-up of fan housing and manifold
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Manifold and fan housing 12V 7cfm fan

The Winning Design

 

   After nearly 45 days of testing, I changed directions a little and stumbled across a design that has turned out to be quite remarkable. Initial testing revealed several qualities that made the configuration ideal. First, it maintained identical and stable temperatures in each egg box. Second, it allowed for the system to achieve temperature gradually (and not exceed it). Third, it incorporated the introduction of fresh air into the system and lastly, it maintained saturation-level humidity. 

   I started with the 32" wire coated rack as a base. I built a frame out of ¾" PVC that fit beneath the wire rack. The heating cable was wrapped around the PVC frame so that it was distributed evenly along the length of the frame. The wire rack prevented the heat cable from being damaged. On the wire rack sit (3) 6 quart 12" x 10" x 4" stainless steel pans filled with water. The stainless steel serves to conduct heat more effectively for even heating and the water served two purposes... to help humidify the incubator, and provide thermal mass to aid in temperature stability.

   One of the things about traditional artificial incubation that's always bothered me is gas exchange. Incubating eggs are metabolizing stored energy and releasing waste gases. Most traditional egg incubation strategies focus on maintaining ideal temperatures and humidity and little attention seems to concentrate on fresh air exchange. On an experimental basis I opted to include some form of fresh air introduction into my incubator designs. For this purpose I opted to use an aquarium air pump (Rena Air model 100). In my early designs the air line ran into the intake manifold where the fresh air mixed with warm internal air before being blown back into the incubator through the exhaust portion of the manifold. Since the manifold proved to be only marginally effective in the testing phase, I reevaluated and bettered my design. I ultimately ran the air line into the center stainless steel water tray and attached an air stone to it. In addition to providing fresh air, the design helped boost the humidity the last several often difficult to achieve percentage points to total saturation.

  The egg boxes sit on styrene egg crate positioned atop a PVC frame that separates it from the stainless steel water pans. It's important that there's a separation between the boxes and the water in order to aid in air flow beneath the egg boxes and to eliminate the presence of hot spots in the incubator. On the underside of the egg crate are fastened two 12V 40mm X 40mm X 20mm CPU fans. I zip tie them to the egg crate and position them to create air flow parallel to the egg crate's surface. They each move approximately 7 cubic feet of air per minute and between the two of them, manage to completely homogenize the air in the incubator... in regard to both temperature and humidity.

   It's important to note that I built this incubator with the intent of using it with a consistent temperature regimen. Because I was anticipating a number of clutches within a narrow timeframe, it wasn't particularly feasible for me to use the 1-5-1, 30.5°C- 31.5°C- 30.0°C regimen. The difference in incubator configuration between the two techniques can be dramatic. A uniform temperature regimen requires consistent ambient incubator air temperatures and the 1-5-1 regimen requires consistent egg surface temperatures. Therefore, the placement of the probe is CRITICAL to achieving an effective incubator. In my final cooler incubator design, the proportional thermostat probe is placed in the airspace underneath the egg boxes where it can sample the fan-mixed air. If the probe is placed inside the egg box (insulated from the ambient air in the incubator), disaster can result. A probe interior to an egg box will send a message to the thermostat to continue providing power to the heat source even after the target temperature is reached inside the incubator. When that happens, the inside of the incubator continues to heat until the egg box reaches the ideal temperature. At that time, the thermostat decreases power to the heating element. Unfortunately, air in the incubator will have already surpassed the target temperature and will continue to heat the egg box until equilibrium is reached. A cyclical increase and decrease in temperature will occur until final equilibrium occurs. My tests indicated that this can mean surpassing ideal temperatures by as much as 4°F and the initiation of a fluctuation cycle that can last as long as two hours after the incubator is closed. In the scenario where the probe is exterior to the egg box, the thermostat decreases power to the heater when the ambient air in the incubator reaches target. The thermostat maintains that temperature and the egg box gradually reaches target, and once equilibrium is reached, the system maintains perfectly.

   I am continuing to test ideas that build upon the design that has thus far been my most successful. Space limitations have kept me from being able to utilize the domed covers in an effective design but work continues... I'll post any effective developments as they occur.

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Wire Shelf and PVC Frame Heat tape wrapped around PVC frame
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Heat tape & PVC frame under plastic-coated wire shelf Stainless Steel Water Pans
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PVC Spacer Frame Fan With Spliced Extensions
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Mounted Fan

Mounted Fan

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Fans and Egg Crate Installed Air Flow Diagram
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Egg Crate and Egg Boxes Incubator With Installed Acrylic Interior Lids
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Incubator Testing Deli-cups in boxes
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nesting container Sarlo in her nest box
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Sarlo laying her eggs 3 March 2005 Sarlo in her nest box 4 March 2005
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Sarlo's eggs 4 March 2005

   

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Last updated: 4 February 2008